Henan Holyrunway Inc.
Main products:Nano Silver,Water-based Zinc Stearate,Nano Tio2 Powder,Nano Tio2 Liquid
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Contact Us
  • Contact Person : Ms. Zhang Kay
  • Company Name : Henan Holyrunway Inc.
  • Tel : 0086-371-86128369
  • Fax : 0086-371-86128369
  • Address : Henan,Zhengzhou,Block Y06,No.11,Changchun Rd.
  • Country/Region : China
  • Zip : 450001

DNA Extraction Kit for Marine Organism

DNA Extraction Kit for Marine Organism
Product Detailed
Our Whole Blood Magnetic DNA Extraction Kit can extract DNA from the samples faster and easier!

Detailed Product Description

Our Magnetic DNA Extraction Kit can extractDNA from the samples faster and easier!

Magnetic Bead DNA Extraction Kit SpeciallyUsed for Marine Organism

1,     Lysis: Use hand drill to get 0.2g clean cancellous substance, mix with0.3ml 1M DDT, grind them into thin mud, then take 0.1g ( about green bean size) out and put it into a 1.5ml EP tube. Add PK 20µL and 300µL lysis buffer.Vibrate evenly and incubate it at 65°C for 1 hour. After taking it out, use atransferpettor ( its mouth wrapped with double layer thin silk net) to get200µL supernatant and put it into another new EP. This can also be done after10min centrifugation. For tissues like muscle and internal organs, they shouldbe cut into small particles first, then do things as above stated.

2,     Bind:  add well-stirred 200µLmagnetic bead liquid into the EP tube, suck it 8 times by using atransferpettor ( suck to 500µL level ) to mix up well, leave it at roomtemperature 25 °C for 6 min, slightly shake the tube several time every 3 min.

3,     Wash: Insert the EP tube into the magnetic rack to allow all themagnetic beads to be absorbed onto the wall of the tube, then fully suck theunnecessary liquid( beads shall not be sucked ).Take the EP tube off the rack,add 600µL wash buffer, and suck it 8 times by using a transferpettor ( suck to700µL level ) to mix up well, leave it there for 30sec. Then reput it onto themagnetic rack to allow all the magnetic beads to be absorbed onto the wall ofthe tube, then fully suck the unnecessary liquid. Repeat process 3 for one moretime. Open the top to allow the beads to dry for 3min at room temperature.

4,    Elute: Get the EP tube, add 100µL elute buffer, and suck it 8 times byusing a transferpettor ( suck to150µL level ) to mix up well. Then put the tubeinto a constant temperature  ( 65°C )water tank to incubate 10 minutes, slightly shake the tube several times every3 min. After 10 min, get the EP tube out, do the magnetic separationimmediately, transfer the supernatant carefully into another new EP tube inorder to do further experiments!

DNA Extraction Kit for Marine Organism



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