Henan Holyrunway Inc.
Main products:Nano Silver,Water-based Zinc Stearate,Nano Tio2 Powder,Nano Tio2 Liquid
Contact Us
  • Contact Person : Ms. Zhang Kay
  • Company Name : Henan Holyrunway Inc.
  • Tel : 0086-371-86128369
  • Fax : 0086-371-86128369
  • Address : Henan,Zhengzhou,Block Y06,No.11,Changchun Rd.
  • Country/Region : China
  • Zip : 450001

Magnetic Bead DNA Extraction Kit of Bone Gene Groups

Magnetic Bead DNA Extraction Kit of Bone Gene Groups
Product Detailed
Our Whole Blood Magnetic DNA Extraction Kit can extract DNA from the samples faster and easier!

Detailed Product Description

Our Magnetic DNA Extraction Kit can extractDNA from the samples faster and easier!

Magnetic Bead DNA Extraction of Bone GeneGroups

1, Carefully drill the clean bone and get0.2g of cancellous substance, and put it into a 1.5ml EP tube. Put 300µL lysisbuffer in. Put the tube into a constant temperature  ( 65°C ) water tank to incubate 30---60minutes.

2, Take the tube out and get 200µL supernatant.Put the supernatant into another new tube and add 300µL bind buffer and 10µLmagnetic bead fluid in. point vibrate 15times to mix up well, leave it at roomtemperature 25 °C for 10 min, reverse it several times every 2----3 min.

3, Magnetic separation:hold the EP tube inthe left hand, hold the rectangular magnet in the right hand and put it on thebottom side of the tube ( 2/3 from tube mouth),and leave it there for half aminute. The beads will be absorbed onto the wall of the tube, then suck all theliquid ( it must be fully-sucked). The magnetic frame inside the kit can alsobe used to do the separation.

4, Put the EP tube on a normal non-magnetictube rack, add in 600µL washing buffer, point vibrate it 15 times to mix upwell ( or suck the liquid slowly 15 times to mix up well), leave the tube stillfor 1 min, then get the EP tube to do the magnetic separation to absorb thebeads onto the wall of tube, then suck all the liquid ( it must befully-sucked).

5, Repeat Process 4 for one time

6, Add 50µL ~ 100µL eluting buffer into theEP tube,  suck the liquid slowly for 15times to mix up well, leave it in a constant temperature ( 65°C ) water tankfor 10min. Slightly shake the EP tube several times every 2---3 minutes.

7, If not for instant use, store the EPtube at 2~8°C. For instant use, rectangular magnet or magnetic frame can beused to do the separation in order to absorb the beads onto the tube wall, thensuck the liquid and transfer it into a new centrifuge tube to do furtherexperiments.

Note: For the DNA extraction of teeth, thismethod can work as a reference.

           For the DNA extraction of hair, it should be hair with follicles 0.5 cmto the hair root. Approximately 10mg , add 30µLPK in, according to need.

           For animal tissues, they should be cut into 1mm particles.

Magnetic Bead DNA Extraction Kit of Bone Gene Groups

Copyright Notice @ 2008-2012 ECVERY Limited and/or its subsidiaries and licensors. All rights reserved.